Journal: Pharmaceutics
Article Title: Synthesis, Formulation and Characterization of Immunotherapeutic Glycosylated Dendrimer/cGAMP Complexes for CD206 Targeted Delivery to M2 Macrophages in Cold Tumors
doi: 10.3390/pharmaceutics14091883
Figure Lengend Snippet: NCs upregulate mRNA expression of pro-inflammatory (M1) genes and reduce the expression of the M2-associated gene Mrc1 in M2 polarized BMDMs. Relative mRNA expression of M2-like macrophage markers ( Mrc1 ( a ) and Arg1 ( b )) and of classically activated (M1-like) macrophage markers ( IL-1b ( c ), STAT1 ( d ) and NOS2 ( e )) in M2 BMDMs treated with corresponding PAMAM NCs (PG3 1/1, PG3 2/1, PG4 1/1 or PG4 2/1), cGAMP (25 µg/condition) or with media. * p < 0.05; ** p < 0.01; **** p < 0.0001.
Article Snippet: Dye: 90 μL of 100 nM and 90 μL of 16 nM Recombinant Mouse Mrc1 (CD206) protein (Leu225 Ser492, His and T7-tagged #Mrc1-7099M Creative BioMart ® ), where the fluorescent signals of CD206 correspond to the typical detection limit of the Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany).
Techniques: Expressing